Columbia Solid Tumor Panel (CSTP)
Purpose
Detect variants in 48 commonly mutated genes in cancer tissue.
CPT Codes
81445, 88381
Methodology
Next Generation Sequencing (NGS) of DNA
Turnaround Time
14 days
Specimen Accepted
Formalin-fixed paraffin embedded (FFPE) or frozen tissue, Cytology specimens in CytoLyt solution, whole blood or bone marrow in EDTA, or DNA extracted from a CLIA laboratory
Genes Tested
AKT1(NM_005163 e3); ALK(NM_004304 e21-25); ARAF(NM_001654 e7); BRAF(NM_004333 e11,15) , CDKN2A(NM_000077 e2); CTNNB1(NM_001904 e3,7,8); CYSLTR2(NM_001308465 e5); DDR2(NM_006182 e17); EGFR(NM_005228 e3,7,12,15,18-21); EIF1AX(NM_001412 e1,2,6); ERBB2(NM_004448 e8,17,19-21); ERBB4(NM_005235 e3,4,6-9,15,23); FBXW7(NM_033632 e5,7-12); FGFR1(NM_023110 e4,7,12,14); FGFR2(NM_000141 e7,9,12); FGFR3(NM_000142 e7,9,14,16,18); GNAQ(NM_002072 e2,4,5); GNAS(NM_000516 e8,9); GNA11(NM_002067 e4-5); H3F3A(NM_002107 e2); HRAS(NM_005343 e2-4); HIST1H3B(NM_003537 e1); IDH1(NM_005896 e4); IDH2(NM_002168 e4); KEAP1(NM_012289.4 e2-6); KIT(NM_000222 e8,9,10,11,13,14,15,17,18); KRAS(NM_004985 e2-4); MAP2K1(NM_002755 e2,3,6,7,11); MET(NM_001127500 e2,14,16,18,19); NRAS(NM_002524 e2-4); NTRK1(NM_002529 e14-15); PDGFRA(NM_006206 e12,14,15,18); PLCB4(NM_000933 e20); PIK3CA(NM_006218 e2,3,5,7,8,10,14,19,21); POLD1(NM_002691 e6-10,15-20,24); POLE(NM_006231 e1,2,9,11,13,14,20,21,25,26); PTEN(NM_000314 e1-9); PTPN11(NM_002834 e3,13); RAC1(NM_006908 e2); RAF1(NM_002880 e7); RET(NM_020975 e10,11,13,15,16); SF3B1(NM_012433 e14); SMAD4(NM_005359 e3-6,8-12); SRSF2(NM_003016 e1); STK11(NM_000455e1-9, full coding sequence); TERT(promoter, NM_198253 5’UTR); TP53(NM_000546 e2,4-10); TSHR(NM_000369 e9-10)