Cancer Whole Exome Sequencing with Transcriptome

Purpose

Detects actionable constitutional changes predisposing to cancer; detects cancer specific missense, indel and copy number changes as well as chimeric RNAs and RNAs with altered expression levels using combination of exome and transcriptome sequencing.

CPT Codes

81415, 81416, 81479

Methodology

Next Generation Sequencing of mRNA and DNA

Turnaround Time

60 days

Specimen

Frozen tissue. A normal control sample (e.q. blood or buccal swab) is required for this testing.

Associated Documents

Cancer Genes (Cosmic):

• COSMIC genes reported on

Coverage Disclaimer:

• WES regions not guaranteed greater than 10 fold
• COSMIC gene exons not guaranteed greater than 30 fold
• Recurrent COSMIC mutations not guaranteed to be covered greater than 30 fold

Secondary Findings

• ACMG Secondary Findings List

Forms

• To inquire about ordering this test please contact: PGMINQUIRY@cumc.columbia.edu
• Cancer Whole Exome Informed Consent
• Insurance Pre-Authorization Form
• Medicare Advance Beneficiary Notice Form
• CWES Letter of Medical Necessity Template