Liza A. Pon, PhD

Profile Headshot

Overview

Academic Appointments

  • Professor of Pathology & Cell Biology

Credentials & Experience

Honors & Awards

1996  Gender Equity Award for promoting a gender-fair environment for the education and training of women physicians and assuring equal opportunity for women and men to study and practice medicine. Awarded by the American Medical Women's Association, Inc.

1998  ad hoc Grant Application Reviewer for Shared Instrumentation Grants (NIH, 7/96); Special Study Section X-84 (NIH, 10/97); Cell Cytology and Physiology (CBY-1, NIHGMS, 2/99); Research Centers in Minority Institution (RCMI, NIH 12/00); General Medicine program projects (NIH, 2/02 and 10/02); National Science Foundation (4/04); Shared Instrumentation Grants (NIH, ZRG1 CB-G (6/09); Shared Instrumentation Grants (NIH, ZRG1 CB-Q (11/09); Enabling Bioanalytical and Imaging Technologies (NIH, 5/13); Cellular Mechainsms of Aging and Development (NIH CMAD 2/14; 2/16); Special Emphasis Panel on Bioengineering Research Partnership (NIH, BRP 10/14); Maximizing Investigators; Research Award (MIRA, NIH, 11/15); ZRG1 CN-T(03), Special Emphasis Panel (SEP) on Shared Confocal Microscopes and Related Imaging Systems (NIH 7/16); Membrane Biology and Protein Processing Study Section (MBPP, NIH, 10/16, 6/17); Deutsche Forschungsgemeinschaft (German Research Foundation; 6/17).

2000–01  Editor, Methods in Cell Biology, volume 65, Mitochondria, Academic Press.

2000  Co-Chair for the Gordon Research Conference, “Plant and Fungal Cytoskeleton”, Andover, NH.

2000–04  Study Section Member, for Cell Development and Function-4 (CDF-4) NIH/NIGMS

2000–05  Editorial Board of Cell Motility and the Cytoskeleton

2001  Gender Equity Award for promoting a gender-fair environment for the education and training of women physicians and assuring equal opportunity for women and men to study and practice medicine. Awarded by the American Medical Women's Association, Inc.

2002  Chair, Gordon Research Conference, “Plant and Fungal Cytoskeleton”, Andover, NH.

2002  The Charles W. Bohmfalk Memorial Prize for Distinguished Contributions to Teaching in recognition of Excellence in Teaching during the Pre-Clinical Years at Columbia University College of Physicians and Surgeons.

2006-07  Editor, Methods in Cell Biology, volume 80, Mitochondria, 2nd Edition, Academic Press and Elsevier.

2006-11  Review Committee Member, Biomedical Research and Training (BRT-A) review committee, NIH/NIGMS

2010-11  Chair, Biomedical Research and Training (BRT-A) Review Committee, NIH/NIGMS

2008-   Editorial Board of the International Journal of Cell Biology.

2013-  Editorial Board of Frontiers in Physiology

2013-  Editorial Board of Microbial Cell

2014  Chair, ZRG1 CB-L, Special Emphasis Panel on Shared Instrumentation: Confocal Microscopy and Imaging, (NIH 9/14)

2015  Co-Organizer, FASEB meeting on “Mitochondrial Biogenesis and Dynamics in Health Disease and Aging”, Palm Beach FL.

2017  Chair, ZRG1 CBJ30, Special Emphasis Panel (SEP) on Shared Instrumentation: High-end and Shared Confocal Microscopes and Related Imaging Systems (NIH 10/17) 

Research

Mitochondria have emerged as central regulators of aging, cell death, energy mobilization, oxidative stress management and calcium homeostasis. Since mitochondria are essential organelles that can only be produced from pre-existing mitochondria, these organelles must be inherited from mother to daughter cells for daughter cell survival. Our research focuses on cytoskeletal dynamics and function in control of mitochondrial motility during inheritance, and the role of this process on cell cycle progression and lifespan control. The model system that we use for most of our studies is the budding yeast, Saccharomyces cerevisae. We are also studying mitochondrial distribution and function in neuronal cell models for the age-associated neuronal degenerative disease, familial Alzheimer's Disease.

Although microtubules serve as tracks for long distance mitochondrial movement in the neuronal axon and other cells, the actin cytoskeleton is used for mitochondrial movement in budding yeast and in specialized regions in polarized mammalian cells including the neuronal synapse and the immunological synapse. Our previous studies revealed mechanisms for bi-directional movement of mitochondria, which relies on actin cables, bundles of F-actin that align along the mother-bud axis, the Arp 2/3 complex, actin polymerization, and actin cable dynamics. Current efforts focus on the mechanism of action of a newly identified Ras-like actin cable regulator, and how actin cable dynamics and function change as a function of age.

There are many checkpoints that regulate cell cycle progression in response to inheritance of the nucleus. Recently, we identified two checkpoints that inhibit cell cycle progression when there are defects in mitochondrial inheritance The mitochondrial inheritance checkpoint that inhibits cell cycle progression at cytokinesis when there are defects in mitochondrial inheritance. This is mediated by a conserved cell cycle checkpoint regulator, the Mitotic Exit Network. The other checkpoint, the mitochondrial DNA (mtDNA) inheritance checkpoint, inhibits progression from G1 to S phase of the cell division cycle when mtDNA in not inherited by daughter cells. This checkpoint is regulated by Rad53p, a component of the DNA Damage Checkpoint signaling pathway. It responds to loss of DNA in the organelle and not to genes encoded by the DNA. Current efforts focus on the sensing mechanism for detecting defects in the inheritance of mitochondria and mtDNA and how this information is transmitted to the signaling pathways that regulate cell cycle progression.

Equally important, we find that the fittest mitochondria, those with the lowest reactive oxygen species (ROS), are preferentially inherited to daughter cells and that mutations that inhibit mitochondrial inheritance produce defects in the inheritance of the fittest mitochondria and premature aging. Recent studies indicate that this occurs in part by cytoskeleton-dependent preferential transport and anchorage of the fittest mitochondria in daughter cells and that increasing mitochondrial quality in daughter cells extends lifespan in budding yeast. Current studies focus on the role of mitochondrial fusion and fission mediators and other quality control mechanisms in this process, and how mitochondrial motility, cytoskeletal interactions and quality control change with age.

Research Interests

  • Cytosketetal control of organelle movements and segregation during cell division

Grants

MITOCHONDRIAL INHERITANCE AND QUALITY CONTROL (Federal Gov)

Apr 1 2017 - Mar 31 2022

THE ROLE OF ACTIN IN CELLULAR AGING (Federal Gov)

Aug 15 2016 - Mar 31 2021

MITOCHONDRIA-CYTOSKELETON COMMUNICATION AND FUNCTION IN AGING (Federal Gov)

Jan 1 2017 - Dec 31 2020

MOLECULAR AND THERAPEUTIC MECHANISMS OF A NEW MODEL OF CONGENITAL MUSCULAR DYSTROPHY (Federal Gov)

Jul 1 2016 - Jun 30 2020

CANCER CENTER SUPPORT GRANT (Federal Gov)

Jul 1 2014 - Jun 30 2019

A NEWLY IDENTIFIED CONGENITAL MUSCULAR DYSTROPHY: MECHANISMS AND INTERVENTIONS (Private)

Aug 1 2015 - Jul 31 2018

MITOCHONDRIAL MOTILITY AND INHERITANCE (Federal Gov)

Sep 1 2013 - Apr 30 2017

MITOCHONDRIAL-CYTOSKELETAL INTERACTIONS AND AGING (Federal Gov)

Sep 1 2011 - May 31 2015

MITOCHONDRIAL LOCALIZATION AND AGING (Private)

Oct 1 2010 - Sep 30 2014

CELL POLARITY AND AGING (Federal Gov)

Aug 1 2009 - Jul 31 2014

SUPER-RESOLUTION STRUCTURED ILLUMINATION MICROSCOPE (Federal Gov)

Jul 1 2013 - Jun 30 2014

RESEARCH COLLABORATION WITH MERCK (Private)

May 27 2009 - Aug 17 2012

MULTIPHOTON CONFOCAL MICROSCOPE (Federal Gov)

Apr 22 2010 - Apr 21 2011

Selected Publications

Hirabayashi Y, Kwon SK, Paek H, Pernice WM, Paul MA, Lee J, Erfani P, Raczkowski A, Petrey DS, Pon LA, Polleux F. ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science. (2017) 358:623-630. PMID: 29097544.  

Pernice WM, Vevea JD, Pon LA. A role for Mfb1p in region-specific anchorage of high-functioning mitochondria and lifespan in Saccharomyces cerevisiae. Nat Commun. (2016) 7:10595. PMID: 26839174; PMCID: PMC4742906.  

Higuchi-Sanabria R, Charalel JK, Viana MP, Garcia EJ, Sing CN, Koenigsberg A,  Swayne TC, Vevea JD, Boldogh IR, Rafelski SM, Pon LA. Mitochondrial anchorage and fusion contribute to mitochondrial inheritance and quality control in the budding yeast Saccharomyces cerevisiae. Mol Biol Cell. (2016) 27:776-87. PMID: 26764088; PMCID: PMC4803304.

Vevea JD, Garcia EJ, Chan RB, Zhou B, Schultz M, Di Paolo G, McCaffery JM, Pon LA. Role for Lipid Droplet Biogenesis and Microlipophagy in Adaptation to Lipid Imbalance in Yeast. Dev Cell. (2015) 35:584-99. PMID: 26651293; PMCID: PMC4679156.

Wolken DM, McInnes J, Pon LA. Aim44p regulates phosphorylation of Hof1p to promote contractile ring closure during cytokinesis in budding yeast. Mol Biol Cell. (2014) 25:753-62. PMID: 24451263; PMCID: PMC3952846.  

Higuchi R, Vevea JD, Swayne TC, Chojnowski R, Hill V, Boldogh IR, Pon LA. Actin dynamics affect mitochondrial quality control and aging in budding yeast. Curr Biol. (2013) 23:2417-22. PMID: 24268413; PMCID: PMC3932488. (This article was highlighted in a Rapid Dispatch in Current Biology and was selected as an F1000Prime paper by the Faculty of 1000.)

Crider DG, García-Rodríguez LJ, Srivastava P, Peraza-Reyes L, Upadhyaya K, Boldogh IR, Pon LA. Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression. J Cell Biol. (2012) 198:793-8. PMID: 22927468; PMCID: PMC3432762. (featured article)

Swayne TC, Zhou C, Boldogh IR, Charalel JK, McFaline-Figueroa JR, Thoms S, Yang C, Leung G, McInnes J, Erdmann R, Pon LA. Role for cER and Mmr1p in anchorage of mitochondria at sites of polarized surface growth in budding yeast.  Curr Biol. (2011) 21:1994-9. PMID: 22119524; PMCID: PMC3237857. (This article was highlighted in a Rapid Dispatch in Current Biology.)

McFaline-Figueroa JR, Vevea J, Swayne TC, Zhou C, Liu C, Leung G, Boldogh IR, Pon LA. Mitochondrial quality control during inheritance is associated with lifespan and mother-daughter age asymmetry in budding yeast. Aging Cell. (2011) 10:885-95 PMID: 21726403; PMCID: PMC3173513.  

Area-Gomez E, de Groof AJ, Boldogh I, Bird TD, Gibson GE, Koehler CM, Yu WH,  Duff KE, Yaffe MP, Pon LA, Schon EA. Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria. Am J Pathol. (2009) 175:1810-6. PMID: 19834068; PMCID: PMC2774047. 

García-Rodríguez LJ, Crider DG, Gay AC, Salanueva IJ, Boldogh IR, Pon LA. Mitochondrial inheritance is required for MEN-regulated cytokinesis in budding yeast. Curr Biol. (2009) 19:1730-5. PMID: 19818621; PMCID: PMC2783229.

Huckaba TM, Lipton T, and Pon LA  Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast. (2006) J. Cell Biol. 175:957-969. PMID: 17178912 (This article is featured in a Rapid Dispatch in Current Biology.)

Huckaba TM, Gay A, Pantalena LF, Yang H-C and Pon LA   Live cell imaging of the assembly, disassembly and actin cable-dependent movement of endosomes and actin patches the yeast Saccharomyces cerevisiae. (2004) J. Cell Biol., 167:519-530. PMID: 15534003 (featured article)