Andrei Tkatchenko, PhD
- Associate Professor of Ophthalmic Sciences (in Opthalmology and Pathology and Cell Biology)
Credentials & Experience
Education & Training
- MS, MD, 1988 Biochemistry/Molecular biology, Pirgov Russian National Research Medical University
- PhD, 1992 Molecular Biology, Engelhardt Institute of Molecular Biology
- Fellowship: Engelhardt Institute of Molecular Biology
- Fellowship: Harvard Univ Medical School
Honors & Awards
1988 Ph.D. Fellowship from the Russian Academy of Sciences
1989 Selected among 70 top graduate students of the USSR in the contest run by Oxford University
1996 Fellowship from Institut National de la Sante et de la Recherche Medicale (INSERM) , France
2001 Selected as Dana/Mahoney Research Fellow by the Harvard Neuroscience Institute
2002 Selected as Dana/Mahoney Research Fellow by the Harvard Neuroscience Institute
The main focus of Dr. Tkatchenko’s research is identification and characterization of genes and genetic networks underlying refractive eye development, as well as studies of genetic variations causing development of refractive errors.
Postnatal refractive eye development is a tightly coordinated process whereby visual input drives ocular growth toward zero refractive error in a process called “emmetropization”. The emmetropization process is regulated by a vision-driven feedback loop in the retina and downstream signaling cascades in other ocular tissues, resulting in correct focal length of the eye (emmetropia). Failure of emmetropization leads to the development of refractive errors, i.e., farsightedness (hyperopia) or nearsightedness (myopia). The prevalence of myopia (the most clinically important refractive error in the human population) has increased from 25% to 44% of the adult population in the United States in the last 30 years, and reached >80% in some parts of Asia. Epidemiological data suggest that low-grade common myopia represents a major risk factor for a number of serious ocular pathologies such as cataract, glaucoma, retinal detachment, and myopic maculopathy, which is comparable to the risks associated with hypertension for stroke and myocardial infarction, and represents the seventh leading cause of blindness. In the U.S., the increasing prevalence of myopia also costs $8.1 billion a year for refractive correction alone, negatively affects self-perception, job and activity choices. It is estimated that 2.5 billion people (1/3 of the world’s population) will be affected by myopia by 2020. Uncorrected refractive errors are the major cause of vision loss (particularly in developing countries) and refractive errors are one of five priority pathological conditions according to the World Health Organization.
Environmental factors, such as nearwork and reading, play important role in the development of common myopia; however, recent human genetic studies and gene expression profiling in animal models of myopia revealed that refractive eye development and myopia are controlled by hundreds of genes and complex genetic networks, which account for more than 70% of variance in refraction.
Dr. Tkatchenko recently developed a mouse model of myopia and demonstrated that mice undergo emmetropization. He also demonstrated that refractive eye development and myopia in the mouse are fundamentally similar to those in other mammals, including humans. Dr. Tkatchenko’s laboratory is using classical mouse genetics, gene-targeted mouse models, and advanced systems genetics approaches to study genes and genetic networks underlying refractive eye development and myopia.
R01EY023839 Tkatchenko (PI) 07/01/2014 – 06/30/2018
Genetics of refractive error development
GENETICS OF REFRACTIVE ERROR DEVELOPMENT IN THE MOUSE MODEL (Federal Gov)
Sep 30 2014 - Aug 31 2018
Tkatchenko AV, Luo X, Tkatchenko TV, Vaz C, Tanavde VM, Maurer-Stroh S, Zauscher S, Gonzalez P, Young TL. Large-scale microRNA expression profiling identifies putative retinal miRNA-mRNA signaling pathways underlying form-deprivation myopia in mice. PLoS One 2016;11:e0162541.
Tkatchenko AV, Tkatchenko TV, Guggenheim JA, Verhoeven VJ, Hysi PG, Wojciechowski R, Singh PK, Kumar A, Thinakaran G, Consortium for Refractive Error and Myopia (CREAM), Williams C. APLP2 regulates refractive error and myopia development in mice and humans. PLOS Genetics 2015; 11(8):e1005432.doi: 10.1371/journal.pgen.1005432.
Bawa G, Tkatchenko TV, Avrutsky I, Tkatchenko AV. Variational analysis of the mouse and rat eye optical parameters. Biomed. Opt. Express 2013; 4: 2585–2595.
Tkatchenko TV, Shen Y, Braun RD, Bawa G, Kumar P, Avrutsky I, Tkatchenko AV. Photopic visual input is necessary for emmetropization in mice. Exp. Eye Res. 2013; 115: 87-95.
Tkatchenko TV, Tkatchenko AV. Ketamine-xylazine anesthesia causes hyperopic refractive shift in mice. J. Neurosci. Methods 2010; 193: 67-71.
Tkatchenko TV, Shen Y, Tkatchenko AV. Mouse experimental myopia has features of primate myopia. Invest. Ophthalmol. Vis. Sci. 2010; 51:1297-1303.
Tkatchenko TV, Shen Y, Tkatchenko AV. Analysis of postnatal eye development in the mouse with high-resolution small animal magnetic resonance imaging. Invest. Ophthalmol. Vis. Sci. 2010; 51: 21-27.
Tkatchenko TV, Moreno-Rodriguez RA, Conway SJ, Markwald RR, Tkatchenko AV. Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease. Physiol. Genomics 2009; 39: 160-168.
Tkatchenko AV, Walsh PA, Tkatchenko TV, Gustincich S, Raviola E. Form deprivation modulates retinal neurogenesis in primate experimental myopia. Proc. Natl. Acad. Sci. USA 2006; 103: 4681 – 4686.
Tkatchenko AV. Whole-mount BrdU staining of proliferating cells by DNase treatment: application to postnatal mammalian retina. BioTechniques 2006; 40: 29 – 32.
Pruett ND, Tkatchenko TV, Jave-Suarez L, Jacobs DF, Potter CS, Tkatchenko AV, Schweizer J, Awgulewitsch A. Krtap16, characterization of a new hair keratin-associated protein (KAP) gene complex on mouse chromosome 16 and evidence for regulation by Hoxc13. J. Biol. Chem. 2004; 279: 51524 – 51533.
Tkatchenko AV, Visconti RP, Shang L, Papenbrock T, Pruett ND, Ito T, Ogawa M, Awgulewitsch A. Overexpression of Hoxc13 in differentiating keratinocytes results in downregulation of a novel hair keratin gene cluster and alopecia. Development 2001; 128: 1547 – 1558.
Cros N, Tkatchenko AV, Pisani DF, Leclerc L, Léger JJ, Marini JF, Dechesne CA. Analyses of altered gene expression in rat soleus muscle atrophied by disuse. J. Cell Biochem. 2001; 83: 508 – 519.
Tkatchenko AV, Pietu G, Cros N, Gannoun-Zaki L, Auffray C, Léger JJ, Dechesne CA. Identification of altered gene expression in skeletal muscles from Duchenne muscular dystrophy patients. Neuromuscul. Disord. 2001; 11: 269 – 277.
Tkatchenko AV, Le Cam G, Léger JJ, Dechesne CA. Large-scale analysis of differential gene expression in the hindlimb muscles and diaphragm of the mdx mouse. Biochim. Biophys. Acta 2000; 1500: 17 –30.
Biltueva LS, Sablina OV, Beklemisheva VR, Shvets Y, Tkachenko A, Dukhanina O, Lushnikova TP, Vorobieva NV, Graphodatsky AS, Kisselev LL. Localization of rat K51 keratin-like locus (Krt10I) to human and animal chromosomes by in situ hybridization. Cytogenet. Cell Genet. 1996; 73: 209 – 213.
Bliskovsky VV, Berdichevsky FB, Tkachenko AV, Belowa ME, Chumakov IM. Coding region of SON gene small transcript contains four areas of complete tandem repeats. Mol. Biol. 1992; 26: 793 – 806.
Tkachenko AV, Buchman VL, Bliskovsky VV, Shvets YP, Kisselev LL. Exons I and VII of the gene (Ker10) encoding human keratin 10 undergo structural rearrangements within repeats. Gene 1992; 116: 245 – 251.
Babaev VR, Belowa ME, Tkachenko AV, Tararak EM, Kazantseva IA, Chumakov IM. The expression of skin-specific gene K51 in the epidermal layer of human skin and in basal cell carcinoma cells. Arch. Dermatol. Res. 1991; 283: 113 –118.