BRAF V600E Mutation Test by Real-time PCR

Purpose: Detect activating mutations of BRAF in various tumors. Select melanoma patients for vemurafenib therapy; aid in the diagnosis of Hairy Cell Leukemia, Langerhans Cell Histiocytosis, and Erdheim Chester Disease; exclude germline MLH1 mutations in colon cancers with MLH1 loss.
CPT Codes: 81210 (88381 if needed)
Methodology: Real-time PCR on DNA obtained from tumor specimens (microdissected to enrich for tumor, if less than 20% tumor nuclei)
Turnaround Time: 15 days (5 working days for metastatic melanoma; “r/o Hairy Cell Leukemia” and “r/o Langerhans Cell Histiocytosis").
Sample tested: Paraffin embedded OR frozen tissue containing the carcinoma in question (see solid tumor requisition for detailed specimen requirements).
Forms: 1. PGM Oncology Requisition
2. Medicare Advance Beneficiary Notice